Recombinant:Article Title: Overexpression of Igf2-derived Mir483 inhibits Igf1 expression and leads to developmental growth restriction and metabolic dysfunction in mice.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-phospho-AKT (Ser 473) Cell Signaling Technology RRID:AB_329825 Polyclonal Goat anti-Mouse IGF-II R&D Systems RRID:AB_2122526 Polyclonal Goat anti-Mouse IGF-I R&D Systems RRID:AB_2248752 Rabbit polyclonal anti-SOD1 Abcam ab183881 Goat anti-Rabbit IgG (HRP) Abcam RRID:AB_955447 Rabbit anti-Goat IgG (HRP) ThermoFisher Scientific RRID:AB_228390 Recombinant Anti-Argonaute-2 antibody Abcam RRID:AB_2713978 Chemicals, peptides, and recombinant proteins Human LR3-IGF1 Preprotech 100-11R3 Protease inhibitors, set III, Calbiochem Merck 535140 Laemmli buffer Merck S3401 Laemmli lysis Merck 38733 RIPA lysis Merck R0278 Immobilon Forte Merck WBLUF Coomasie-250 staining Merck 1154440025 Critical commercial assays In-Fusion HD Cloning Plus kit Takara Bio 638909 RNeasy Plus Mini Kit Qiagen 74134 mirVana kit ThermoFisher Scientific AM1560 miRNeasy Mini kit Qiagen 217004 RNase-Free DNase ThermoFisher Scientific EN0521 RNA 6000 Pico kit Agilent 5067–1513 RNA 6000 Nano kit Agilent 5067–1511 DNA 12000 Kit Agilent 5067–1508 TruSeq Stranded mRNA HT Sample Prep Kit Illumina 20040532 TruSeq Stranded mRNA LT Sample Prep Kit Illumina RS-122-2101 TruSeq Small RNA Library Preparation Kit Illumina RS-200-0012 RevertAid RT Reverse Transcription Kit ThermoFisher Scientific K1622 TaqMan MicroRNA Reverse Transcription Kit ThermoFisher Scientific 4366596 SYBR Green JumpStart Taq Ready Mix Sigma Aldrich (Merck) S4438 TaqPath ProAmp Master Mix ThermoFisher Scientific A30866 TMTsixplex Isobaric Mass Tagging Kit ThermoFisher Scientific 90064 BCA assay ThermoFisher Scientific 23221 IGF-1 Quantikine ELISA kit Biotechne MG100 Mouse/rat growth hormone ELISA kit Millipore EZRMGH-45K Mouse IGF-II DuoSet ELISA kit R&D Systems DY792 Mouse/Rat Insulin Assay Kit Mercodia 10-1247-01 Roche’s Free Fatty Acid Kit Sigma Aldrich (Merck) 11383175001 QuickChange Site-Directed Mutagenesis kit Agilent Technologies 200518 Dual-Luciferase Reporter Assay System Promega E1910 Deposited data mRNA-seq obtained in E18.5 livers of the Mir483KO mouse model This paper GEO: GSE256302 (Continued on next page) 20 Cell Reports 43, 114750, September 24, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER mRNA-seq obtained in E10.5 embryos of the Mir4835C mouse model This paper GEO: GSE256306 mRNA-seq obtained in livers of 15-week-old Mir483KO mutants and littermate controls This paper GEO: GSE256304 miR-seq obtained in E18.5 livers of the Mir483KO mouse model This paper GEO: GSE256305 TMT mass spectrometry data obtained in E10.5 embryos of the Mir4835C mouse model This paper PXD051516 Experimental models: Cell lines E14 129ola male ES cells Hooper et al.63 RRID:CVCL_C320 Targeted JM8.F6 ES cells, International Knockout Mouse Consortium Project Design ID: 49935 Prosser et al.64 RRID:CVCL_J961 E14Tg2A.4 ES cells Smith and Hooper65 RRID:CVCL_Y481 Experimental models: Organisms/strains Mouse Igf2D(P1-P3) This paper N/A Mouse H19D13 Leighton et al.30 The Jackson Laboratory (Stock #036471) Mouse Igf2LacZ Murrell et al. 200135 N/A Mouse CMVCre Schwenk et al.66 The Jackson Laboratory (Stock #006054) Mouse Igf2fl/fl Hammerle et al.34 N/A Mouse Mir483KO This paper N/A Mouse Mir4835C This paper N/A Mouse iTgMir483 This paper N/A Oligonucleotides Primers used for genotyping, see Table S7 This paper Sigma Aldrich (Merck) TaqMan assays used for RT-qPCR, see Table S7 This paper Thermo Fisher Scientific Primers used for RT-qPCR, see Table S7 This paper Sigma Aldrich (Merck) Recombinant DNA pCAG-Cre plasmid Matsuda and Cepko67 RRID:Addgene_13775 pMA_F3NeoLoxPPolyFRT vector Prosser et al.64 N/A pPGKFLPobpA plasmid Raymond and Soriano68 RRID:Addgene_13793 pTET-BigT plasmid Mao et al.69 N/A pROSA26PAS plasmid Mao et al.69 N/A pGL3-Basic Promega (#E1751) RRID:Addgene_40342 Software and algorithms DGE analysis of the effect of Mir-483 on mouse livers This paper https://doi.org/10.5281/zenodo.
Lysis:Article Title: Overexpression of Igf2-derived Mir483 inhibits Igf1 expression and leads to developmental growth restriction and metabolic dysfunction in mice.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-phospho-AKT (Ser 473) Cell Signaling Technology RRID:AB_329825 Polyclonal Goat anti-Mouse IGF-II R&D Systems RRID:AB_2122526 Polyclonal Goat anti-Mouse IGF-I R&D Systems RRID:AB_2248752 Rabbit polyclonal anti-SOD1 Abcam ab183881 Goat anti-Rabbit IgG (HRP) Abcam RRID:AB_955447 Rabbit anti-Goat IgG (HRP) ThermoFisher Scientific RRID:AB_228390 Recombinant Anti-Argonaute-2 antibody Abcam RRID:AB_2713978 Chemicals, peptides, and recombinant proteins Human LR3-IGF1 Preprotech 100-11R3 Protease inhibitors, set III, Calbiochem Merck 535140 Laemmli buffer Merck S3401 Laemmli lysis Merck 38733 RIPA lysis Merck R0278 Immobilon Forte Merck WBLUF Coomasie-250 staining Merck 1154440025 Critical commercial assays In-Fusion HD Cloning Plus kit Takara Bio 638909 RNeasy Plus Mini Kit Qiagen 74134 mirVana kit ThermoFisher Scientific AM1560 miRNeasy Mini kit Qiagen 217004 RNase-Free DNase ThermoFisher Scientific EN0521 RNA 6000 Pico kit Agilent 5067–1513 RNA 6000 Nano kit Agilent 5067–1511 DNA 12000 Kit Agilent 5067–1508 TruSeq Stranded mRNA HT Sample Prep Kit Illumina 20040532 TruSeq Stranded mRNA LT Sample Prep Kit Illumina RS-122-2101 TruSeq Small RNA Library Preparation Kit Illumina RS-200-0012 RevertAid RT Reverse Transcription Kit ThermoFisher Scientific K1622 TaqMan MicroRNA Reverse Transcription Kit ThermoFisher Scientific 4366596 SYBR Green JumpStart Taq Ready Mix Sigma Aldrich (Merck) S4438 TaqPath ProAmp Master Mix ThermoFisher Scientific A30866 TMTsixplex Isobaric Mass Tagging Kit ThermoFisher Scientific 90064 BCA assay ThermoFisher Scientific 23221 IGF-1 Quantikine ELISA kit Biotechne MG100 Mouse/rat growth hormone ELISA kit Millipore EZRMGH-45K Mouse IGF-II DuoSet ELISA kit R&D Systems DY792 Mouse/Rat Insulin Assay Kit Mercodia 10-1247-01 Roche’s Free Fatty Acid Kit Sigma Aldrich (Merck) 11383175001 QuickChange Site-Directed Mutagenesis kit Agilent Technologies 200518 Dual-Luciferase Reporter Assay System Promega E1910 Deposited data mRNA-seq obtained in E18.5 livers of the Mir483KO mouse model This paper GEO: GSE256302 (Continued on next page) 20 Cell Reports 43, 114750, September 24, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER mRNA-seq obtained in E10.5 embryos of the Mir4835C mouse model This paper GEO: GSE256306 mRNA-seq obtained in livers of 15-week-old Mir483KO mutants and littermate controls This paper GEO: GSE256304 miR-seq obtained in E18.5 livers of the Mir483KO mouse model This paper GEO: GSE256305 TMT mass spectrometry data obtained in E10.5 embryos of the Mir4835C mouse model This paper PXD051516 Experimental models: Cell lines E14 129ola male ES cells Hooper et al.63 RRID:CVCL_C320 Targeted JM8.F6 ES cells, International Knockout Mouse Consortium Project Design ID: 49935 Prosser et al.64 RRID:CVCL_J961 E14Tg2A.4 ES cells Smith and Hooper65 RRID:CVCL_Y481 Experimental models: Organisms/strains Mouse Igf2D(P1-P3) This paper N/A Mouse H19D13 Leighton et al.30 The Jackson Laboratory (Stock #036471) Mouse Igf2LacZ Murrell et al. 200135 N/A Mouse CMVCre Schwenk et al.66 The Jackson Laboratory (Stock #006054) Mouse Igf2fl/fl Hammerle et al.34 N/A Mouse Mir483KO This paper N/A Mouse Mir4835C This paper N/A Mouse iTgMir483 This paper N/A Oligonucleotides Primers used for genotyping, see Table S7 This paper Sigma Aldrich (Merck) TaqMan assays used for RT-qPCR, see Table S7 This paper Thermo Fisher Scientific Primers used for RT-qPCR, see Table S7 This paper Sigma Aldrich (Merck) Recombinant DNA pCAG-Cre plasmid Matsuda and Cepko67 RRID:Addgene_13775 pMA_F3NeoLoxPPolyFRT vector Prosser et al.64 N/A pPGKFLPobpA plasmid Raymond and Soriano68 RRID:Addgene_13793 pTET-BigT plasmid Mao et al.69 N/A pROSA26PAS plasmid Mao et al.69 N/A pGL3-Basic Promega (#E1751) RRID:Addgene_40342 Software and algorithms DGE analysis of the effect of Mir-483 on mouse livers This paper https://doi.org/10.5281/zenodo.
Staining:Article Title: Overexpression of Igf2-derived Mir483 inhibits Igf1 expression and leads to developmental growth restriction and metabolic dysfunction in mice.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-phospho-AKT (Ser 473) Cell Signaling Technology RRID:AB_329825 Polyclonal Goat anti-Mouse IGF-II R&D Systems RRID:AB_2122526 Polyclonal Goat anti-Mouse IGF-I R&D Systems RRID:AB_2248752 Rabbit polyclonal anti-SOD1 Abcam ab183881 Goat anti-Rabbit IgG (HRP) Abcam RRID:AB_955447 Rabbit anti-Goat IgG (HRP) ThermoFisher Scientific RRID:AB_228390 Recombinant Anti-Argonaute-2 antibody Abcam RRID:AB_2713978 Chemicals, peptides, and recombinant proteins Human LR3-IGF1 Preprotech 100-11R3 Protease inhibitors, set III, Calbiochem Merck 535140 Laemmli buffer Merck S3401 Laemmli lysis Merck 38733 RIPA lysis Merck R0278 Immobilon Forte Merck WBLUF Coomasie-250 staining Merck 1154440025 Critical commercial assays In-Fusion HD Cloning Plus kit Takara Bio 638909 RNeasy Plus Mini Kit Qiagen 74134 mirVana kit ThermoFisher Scientific AM1560 miRNeasy Mini kit Qiagen 217004 RNase-Free DNase ThermoFisher Scientific EN0521 RNA 6000 Pico kit Agilent 5067–1513 RNA 6000 Nano kit Agilent 5067–1511 DNA 12000 Kit Agilent 5067–1508 TruSeq Stranded mRNA HT Sample Prep Kit Illumina 20040532 TruSeq Stranded mRNA LT Sample Prep Kit Illumina RS-122-2101 TruSeq Small RNA Library Preparation Kit Illumina RS-200-0012 RevertAid RT Reverse Transcription Kit ThermoFisher Scientific K1622 TaqMan MicroRNA Reverse Transcription Kit ThermoFisher Scientific 4366596 SYBR Green JumpStart Taq Ready Mix Sigma Aldrich (Merck) S4438 TaqPath ProAmp Master Mix ThermoFisher Scientific A30866 TMTsixplex Isobaric Mass Tagging Kit ThermoFisher Scientific 90064 BCA assay ThermoFisher Scientific 23221 IGF-1 Quantikine ELISA kit Biotechne MG100 Mouse/rat growth hormone ELISA kit Millipore EZRMGH-45K Mouse IGF-II DuoSet ELISA kit R&D Systems DY792 Mouse/Rat Insulin Assay Kit Mercodia 10-1247-01 Roche’s Free Fatty Acid Kit Sigma Aldrich (Merck) 11383175001 QuickChange Site-Directed Mutagenesis kit Agilent Technologies 200518 Dual-Luciferase Reporter Assay System Promega E1910 Deposited data mRNA-seq obtained in E18.5 livers of the Mir483KO mouse model This paper GEO: GSE256302 (Continued on next page) 20 Cell Reports 43, 114750, September 24, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER mRNA-seq obtained in E10.5 embryos of the Mir4835C mouse model This paper GEO: GSE256306 mRNA-seq obtained in livers of 15-week-old Mir483KO mutants and littermate controls This paper GEO: GSE256304 miR-seq obtained in E18.5 livers of the Mir483KO mouse model This paper GEO: GSE256305 TMT mass spectrometry data obtained in E10.5 embryos of the Mir4835C mouse model This paper PXD051516 Experimental models: Cell lines E14 129ola male ES cells Hooper et al.63 RRID:CVCL_C320 Targeted JM8.F6 ES cells, International Knockout Mouse Consortium Project Design ID: 49935 Prosser et al.64 RRID:CVCL_J961 E14Tg2A.4 ES cells Smith and Hooper65 RRID:CVCL_Y481 Experimental models: Organisms/strains Mouse Igf2D(P1-P3) This paper N/A Mouse H19D13 Leighton et al.30 The Jackson Laboratory (Stock #036471) Mouse Igf2LacZ Murrell et al. 200135 N/A Mouse CMVCre Schwenk et al.66 The Jackson Laboratory (Stock #006054) Mouse Igf2fl/fl Hammerle et al.34 N/A Mouse Mir483KO This paper N/A Mouse Mir4835C This paper N/A Mouse iTgMir483 This paper N/A Oligonucleotides Primers used for genotyping, see Table S7 This paper Sigma Aldrich (Merck) TaqMan assays used for RT-qPCR, see Table S7 This paper Thermo Fisher Scientific Primers used for RT-qPCR, see Table S7 This paper Sigma Aldrich (Merck) Recombinant DNA pCAG-Cre plasmid Matsuda and Cepko67 RRID:Addgene_13775 pMA_F3NeoLoxPPolyFRT vector Prosser et al.64 N/A pPGKFLPobpA plasmid Raymond and Soriano68 RRID:Addgene_13793 pTET-BigT plasmid Mao et al.69 N/A pROSA26PAS plasmid Mao et al.69 N/A pGL3-Basic Promega (#E1751) RRID:Addgene_40342 Software and algorithms DGE analysis of the effect of Mir-483 on mouse livers This paper https://doi.org/10.5281/zenodo.
Cloning:Article Title: Overexpression of Igf2-derived Mir483 inhibits Igf1 expression and leads to developmental growth restriction and metabolic dysfunction in mice.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-phospho-AKT (Ser 473) Cell Signaling Technology RRID:AB_329825 Polyclonal Goat anti-Mouse IGF-II R&D Systems RRID:AB_2122526 Polyclonal Goat anti-Mouse IGF-I R&D Systems RRID:AB_2248752 Rabbit polyclonal anti-SOD1 Abcam ab183881 Goat anti-Rabbit IgG (HRP) Abcam RRID:AB_955447 Rabbit anti-Goat IgG (HRP) ThermoFisher Scientific RRID:AB_228390 Recombinant Anti-Argonaute-2 antibody Abcam RRID:AB_2713978 Chemicals, peptides, and recombinant proteins Human LR3-IGF1 Preprotech 100-11R3 Protease inhibitors, set III, Calbiochem Merck 535140 Laemmli buffer Merck S3401 Laemmli lysis Merck 38733 RIPA lysis Merck R0278 Immobilon Forte Merck WBLUF Coomasie-250 staining Merck 1154440025 Critical commercial assays In-Fusion HD Cloning Plus kit Takara Bio 638909 RNeasy Plus Mini Kit Qiagen 74134 mirVana kit ThermoFisher Scientific AM1560 miRNeasy Mini kit Qiagen 217004 RNase-Free DNase ThermoFisher Scientific EN0521 RNA 6000 Pico kit Agilent 5067–1513 RNA 6000 Nano kit Agilent 5067–1511 DNA 12000 Kit Agilent 5067–1508 TruSeq Stranded mRNA HT Sample Prep Kit Illumina 20040532 TruSeq Stranded mRNA LT Sample Prep Kit Illumina RS-122-2101 TruSeq Small RNA Library Preparation Kit Illumina RS-200-0012 RevertAid RT Reverse Transcription Kit ThermoFisher Scientific K1622 TaqMan MicroRNA Reverse Transcription Kit ThermoFisher Scientific 4366596 SYBR Green JumpStart Taq Ready Mix Sigma Aldrich (Merck) S4438 TaqPath ProAmp Master Mix ThermoFisher Scientific A30866 TMTsixplex Isobaric Mass Tagging Kit ThermoFisher Scientific 90064 BCA assay ThermoFisher Scientific 23221 IGF-1 Quantikine ELISA kit Biotechne MG100 Mouse/rat growth hormone ELISA kit Millipore EZRMGH-45K Mouse IGF-II DuoSet ELISA kit R&D Systems DY792 Mouse/Rat Insulin Assay Kit Mercodia 10-1247-01 Roche’s Free Fatty Acid Kit Sigma Aldrich (Merck) 11383175001 QuickChange Site-Directed Mutagenesis kit Agilent Technologies 200518 Dual-Luciferase Reporter Assay System Promega E1910 Deposited data mRNA-seq obtained in E18.5 livers of the Mir483KO mouse model This paper GEO: GSE256302 (Continued on next page) 20 Cell Reports 43, 114750, September 24, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER mRNA-seq obtained in E10.5 embryos of the Mir4835C mouse model This paper GEO: GSE256306 mRNA-seq obtained in livers of 15-week-old Mir483KO mutants and littermate controls This paper GEO: GSE256304 miR-seq obtained in E18.5 livers of the Mir483KO mouse model This paper GEO: GSE256305 TMT mass spectrometry data obtained in E10.5 embryos of the Mir4835C mouse model This paper PXD051516 Experimental models: Cell lines E14 129ola male ES cells Hooper et al.63 RRID:CVCL_C320 Targeted JM8.F6 ES cells, International Knockout Mouse Consortium Project Design ID: 49935 Prosser et al.64 RRID:CVCL_J961 E14Tg2A.4 ES cells Smith and Hooper65 RRID:CVCL_Y481 Experimental models: Organisms/strains Mouse Igf2D(P1-P3) This paper N/A Mouse H19D13 Leighton et al.30 The Jackson Laboratory (Stock #036471) Mouse Igf2LacZ Murrell et al. 200135 N/A Mouse CMVCre Schwenk et al.66 The Jackson Laboratory (Stock #006054) Mouse Igf2fl/fl Hammerle et al.34 N/A Mouse Mir483KO This paper N/A Mouse Mir4835C This paper N/A Mouse iTgMir483 This paper N/A Oligonucleotides Primers used for genotyping, see Table S7 This paper Sigma Aldrich (Merck) TaqMan assays used for RT-qPCR, see Table S7 This paper Thermo Fisher Scientific Primers used for RT-qPCR, see Table S7 This paper Sigma Aldrich (Merck) Recombinant DNA pCAG-Cre plasmid Matsuda and Cepko67 RRID:Addgene_13775 pMA_F3NeoLoxPPolyFRT vector Prosser et al.64 N/A pPGKFLPobpA plasmid Raymond and Soriano68 RRID:Addgene_13793 pTET-BigT plasmid Mao et al.69 N/A pROSA26PAS plasmid Mao et al.69 N/A pGL3-Basic Promega (#E1751) RRID:Addgene_40342 Software and algorithms DGE analysis of the effect of Mir-483 on mouse livers This paper https://doi.org/10.5281/zenodo.
Sample Prep:Article Title: Overexpression of Igf2-derived Mir483 inhibits Igf1 expression and leads to developmental growth restriction and metabolic dysfunction in mice.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-phospho-AKT (Ser 473) Cell Signaling Technology RRID:AB_329825 Polyclonal Goat anti-Mouse IGF-II R&D Systems RRID:AB_2122526 Polyclonal Goat anti-Mouse IGF-I R&D Systems RRID:AB_2248752 Rabbit polyclonal anti-SOD1 Abcam ab183881 Goat anti-Rabbit IgG (HRP) Abcam RRID:AB_955447 Rabbit anti-Goat IgG (HRP) ThermoFisher Scientific RRID:AB_228390 Recombinant Anti-Argonaute-2 antibody Abcam RRID:AB_2713978 Chemicals, peptides, and recombinant proteins Human LR3-IGF1 Preprotech 100-11R3 Protease inhibitors, set III, Calbiochem Merck 535140 Laemmli buffer Merck S3401 Laemmli lysis Merck 38733 RIPA lysis Merck R0278 Immobilon Forte Merck WBLUF Coomasie-250 staining Merck 1154440025 Critical commercial assays In-Fusion HD Cloning Plus kit Takara Bio 638909 RNeasy Plus Mini Kit Qiagen 74134 mirVana kit ThermoFisher Scientific AM1560 miRNeasy Mini kit Qiagen 217004 RNase-Free DNase ThermoFisher Scientific EN0521 RNA 6000 Pico kit Agilent 5067–1513 RNA 6000 Nano kit Agilent 5067–1511 DNA 12000 Kit Agilent 5067–1508 TruSeq Stranded mRNA HT Sample Prep Kit Illumina 20040532 TruSeq Stranded mRNA LT Sample Prep Kit Illumina RS-122-2101 TruSeq Small RNA Library Preparation Kit Illumina RS-200-0012 RevertAid RT Reverse Transcription Kit ThermoFisher Scientific K1622 TaqMan MicroRNA Reverse Transcription Kit ThermoFisher Scientific 4366596 SYBR Green JumpStart Taq Ready Mix Sigma Aldrich (Merck) S4438 TaqPath ProAmp Master Mix ThermoFisher Scientific A30866 TMTsixplex Isobaric Mass Tagging Kit ThermoFisher Scientific 90064 BCA assay ThermoFisher Scientific 23221 IGF-1 Quantikine ELISA kit Biotechne MG100 Mouse/rat growth hormone ELISA kit Millipore EZRMGH-45K Mouse IGF-II DuoSet ELISA kit R&D Systems DY792 Mouse/Rat Insulin Assay Kit Mercodia 10-1247-01 Roche’s Free Fatty Acid Kit Sigma Aldrich (Merck) 11383175001 QuickChange Site-Directed Mutagenesis kit Agilent Technologies 200518 Dual-Luciferase Reporter Assay System Promega E1910 Deposited data mRNA-seq obtained in E18.5 livers of the Mir483KO mouse model This paper GEO: GSE256302 (Continued on next page) 20 Cell Reports 43, 114750, September 24, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER mRNA-seq obtained in E10.5 embryos of the Mir4835C mouse model This paper GEO: GSE256306 mRNA-seq obtained in livers of 15-week-old Mir483KO mutants and littermate controls This paper GEO: GSE256304 miR-seq obtained in E18.5 livers of the Mir483KO mouse model This paper GEO: GSE256305 TMT mass spectrometry data obtained in E10.5 embryos of the Mir4835C mouse model This paper PXD051516 Experimental models: Cell lines E14 129ola male ES cells Hooper et al.63 RRID:CVCL_C320 Targeted JM8.F6 ES cells, International Knockout Mouse Consortium Project Design ID: 49935 Prosser et al.64 RRID:CVCL_J961 E14Tg2A.4 ES cells Smith and Hooper65 RRID:CVCL_Y481 Experimental models: Organisms/strains Mouse Igf2D(P1-P3) This paper N/A Mouse H19D13 Leighton et al.30 The Jackson Laboratory (Stock #036471) Mouse Igf2LacZ Murrell et al. 200135 N/A Mouse CMVCre Schwenk et al.66 The Jackson Laboratory (Stock #006054) Mouse Igf2fl/fl Hammerle et al.34 N/A Mouse Mir483KO This paper N/A Mouse Mir4835C This paper N/A Mouse iTgMir483 This paper N/A Oligonucleotides Primers used for genotyping, see Table S7 This paper Sigma Aldrich (Merck) TaqMan assays used for RT-qPCR, see Table S7 This paper Thermo Fisher Scientific Primers used for RT-qPCR, see Table S7 This paper Sigma Aldrich (Merck) Recombinant DNA pCAG-Cre plasmid Matsuda and Cepko67 RRID:Addgene_13775 pMA_F3NeoLoxPPolyFRT vector Prosser et al.64 N/A pPGKFLPobpA plasmid Raymond and Soriano68 RRID:Addgene_13793 pTET-BigT plasmid Mao et al.69 N/A pROSA26PAS plasmid Mao et al.69 N/A pGL3-Basic Promega (#E1751) RRID:Addgene_40342 Software and algorithms DGE analysis of the effect of Mir-483 on mouse livers This paper https://doi.org/10.5281/zenodo.
RNA Library Preparation:Article Title: Overexpression of Igf2-derived Mir483 inhibits Igf1 expression and leads to developmental growth restriction and metabolic dysfunction in mice.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-phospho-AKT (Ser 473) Cell Signaling Technology RRID:AB_329825 Polyclonal Goat anti-Mouse IGF-II R&D Systems RRID:AB_2122526 Polyclonal Goat anti-Mouse IGF-I R&D Systems RRID:AB_2248752 Rabbit polyclonal anti-SOD1 Abcam ab183881 Goat anti-Rabbit IgG (HRP) Abcam RRID:AB_955447 Rabbit anti-Goat IgG (HRP) ThermoFisher Scientific RRID:AB_228390 Recombinant Anti-Argonaute-2 antibody Abcam RRID:AB_2713978 Chemicals, peptides, and recombinant proteins Human LR3-IGF1 Preprotech 100-11R3 Protease inhibitors, set III, Calbiochem Merck 535140 Laemmli buffer Merck S3401 Laemmli lysis Merck 38733 RIPA lysis Merck R0278 Immobilon Forte Merck WBLUF Coomasie-250 staining Merck 1154440025 Critical commercial assays In-Fusion HD Cloning Plus kit Takara Bio 638909 RNeasy Plus Mini Kit Qiagen 74134 mirVana kit ThermoFisher Scientific AM1560 miRNeasy Mini kit Qiagen 217004 RNase-Free DNase ThermoFisher Scientific EN0521 RNA 6000 Pico kit Agilent 5067–1513 RNA 6000 Nano kit Agilent 5067–1511 DNA 12000 Kit Agilent 5067–1508 TruSeq Stranded mRNA HT Sample Prep Kit Illumina 20040532 TruSeq Stranded mRNA LT Sample Prep Kit Illumina RS-122-2101 TruSeq Small RNA Library Preparation Kit Illumina RS-200-0012 RevertAid RT Reverse Transcription Kit ThermoFisher Scientific K1622 TaqMan MicroRNA Reverse Transcription Kit ThermoFisher Scientific 4366596 SYBR Green JumpStart Taq Ready Mix Sigma Aldrich (Merck) S4438 TaqPath ProAmp Master Mix ThermoFisher Scientific A30866 TMTsixplex Isobaric Mass Tagging Kit ThermoFisher Scientific 90064 BCA assay ThermoFisher Scientific 23221 IGF-1 Quantikine ELISA kit Biotechne MG100 Mouse/rat growth hormone ELISA kit Millipore EZRMGH-45K Mouse IGF-II DuoSet ELISA kit R&D Systems DY792 Mouse/Rat Insulin Assay Kit Mercodia 10-1247-01 Roche’s Free Fatty Acid Kit Sigma Aldrich (Merck) 11383175001 QuickChange Site-Directed Mutagenesis kit Agilent Technologies 200518 Dual-Luciferase Reporter Assay System Promega E1910 Deposited data mRNA-seq obtained in E18.5 livers of the Mir483KO mouse model This paper GEO: GSE256302 (Continued on next page) 20 Cell Reports 43, 114750, September 24, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER mRNA-seq obtained in E10.5 embryos of the Mir4835C mouse model This paper GEO: GSE256306 mRNA-seq obtained in livers of 15-week-old Mir483KO mutants and littermate controls This paper GEO: GSE256304 miR-seq obtained in E18.5 livers of the Mir483KO mouse model This paper GEO: GSE256305 TMT mass spectrometry data obtained in E10.5 embryos of the Mir4835C mouse model This paper PXD051516 Experimental models: Cell lines E14 129ola male ES cells Hooper et al.63 RRID:CVCL_C320 Targeted JM8.F6 ES cells, International Knockout Mouse Consortium Project Design ID: 49935 Prosser et al.64 RRID:CVCL_J961 E14Tg2A.4 ES cells Smith and Hooper65 RRID:CVCL_Y481 Experimental models: Organisms/strains Mouse Igf2D(P1-P3) This paper N/A Mouse H19D13 Leighton et al.30 The Jackson Laboratory (Stock #036471) Mouse Igf2LacZ Murrell et al. 200135 N/A Mouse CMVCre Schwenk et al.66 The Jackson Laboratory (Stock #006054) Mouse Igf2fl/fl Hammerle et al.34 N/A Mouse Mir483KO This paper N/A Mouse Mir4835C This paper N/A Mouse iTgMir483 This paper N/A Oligonucleotides Primers used for genotyping, see Table S7 This paper Sigma Aldrich (Merck) TaqMan assays used for RT-qPCR, see Table S7 This paper Thermo Fisher Scientific Primers used for RT-qPCR, see Table S7 This paper Sigma Aldrich (Merck) Recombinant DNA pCAG-Cre plasmid Matsuda and Cepko67 RRID:Addgene_13775 pMA_F3NeoLoxPPolyFRT vector Prosser et al.64 N/A pPGKFLPobpA plasmid Raymond and Soriano68 RRID:Addgene_13793 pTET-BigT plasmid Mao et al.69 N/A pROSA26PAS plasmid Mao et al.69 N/A pGL3-Basic Promega (#E1751) RRID:Addgene_40342 Software and algorithms DGE analysis of the effect of Mir-483 on mouse livers This paper https://doi.org/10.5281/zenodo.
Reverse Transcription:Article Title: Overexpression of Igf2-derived Mir483 inhibits Igf1 expression and leads to developmental growth restriction and metabolic dysfunction in mice.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-phospho-AKT (Ser 473) Cell Signaling Technology RRID:AB_329825 Polyclonal Goat anti-Mouse IGF-II R&D Systems RRID:AB_2122526 Polyclonal Goat anti-Mouse IGF-I R&D Systems RRID:AB_2248752 Rabbit polyclonal anti-SOD1 Abcam ab183881 Goat anti-Rabbit IgG (HRP) Abcam RRID:AB_955447 Rabbit anti-Goat IgG (HRP) ThermoFisher Scientific RRID:AB_228390 Recombinant Anti-Argonaute-2 antibody Abcam RRID:AB_2713978 Chemicals, peptides, and recombinant proteins Human LR3-IGF1 Preprotech 100-11R3 Protease inhibitors, set III, Calbiochem Merck 535140 Laemmli buffer Merck S3401 Laemmli lysis Merck 38733 RIPA lysis Merck R0278 Immobilon Forte Merck WBLUF Coomasie-250 staining Merck 1154440025 Critical commercial assays In-Fusion HD Cloning Plus kit Takara Bio 638909 RNeasy Plus Mini Kit Qiagen 74134 mirVana kit ThermoFisher Scientific AM1560 miRNeasy Mini kit Qiagen 217004 RNase-Free DNase ThermoFisher Scientific EN0521 RNA 6000 Pico kit Agilent 5067–1513 RNA 6000 Nano kit Agilent 5067–1511 DNA 12000 Kit Agilent 5067–1508 TruSeq Stranded mRNA HT Sample Prep Kit Illumina 20040532 TruSeq Stranded mRNA LT Sample Prep Kit Illumina RS-122-2101 TruSeq Small RNA Library Preparation Kit Illumina RS-200-0012 RevertAid RT Reverse Transcription Kit ThermoFisher Scientific K1622 TaqMan MicroRNA Reverse Transcription Kit ThermoFisher Scientific 4366596 SYBR Green JumpStart Taq Ready Mix Sigma Aldrich (Merck) S4438 TaqPath ProAmp Master Mix ThermoFisher Scientific A30866 TMTsixplex Isobaric Mass Tagging Kit ThermoFisher Scientific 90064 BCA assay ThermoFisher Scientific 23221 IGF-1 Quantikine ELISA kit Biotechne MG100 Mouse/rat growth hormone ELISA kit Millipore EZRMGH-45K Mouse IGF-II DuoSet ELISA kit R&D Systems DY792 Mouse/Rat Insulin Assay Kit Mercodia 10-1247-01 Roche’s Free Fatty Acid Kit Sigma Aldrich (Merck) 11383175001 QuickChange Site-Directed Mutagenesis kit Agilent Technologies 200518 Dual-Luciferase Reporter Assay System Promega E1910 Deposited data mRNA-seq obtained in E18.5 livers of the Mir483KO mouse model This paper GEO: GSE256302 (Continued on next page) 20 Cell Reports 43, 114750, September 24, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER mRNA-seq obtained in E10.5 embryos of the Mir4835C mouse model This paper GEO: GSE256306 mRNA-seq obtained in livers of 15-week-old Mir483KO mutants and littermate controls This paper GEO: GSE256304 miR-seq obtained in E18.5 livers of the Mir483KO mouse model This paper GEO: GSE256305 TMT mass spectrometry data obtained in E10.5 embryos of the Mir4835C mouse model This paper PXD051516 Experimental models: Cell lines E14 129ola male ES cells Hooper et al.63 RRID:CVCL_C320 Targeted JM8.F6 ES cells, International Knockout Mouse Consortium Project Design ID: 49935 Prosser et al.64 RRID:CVCL_J961 E14Tg2A.4 ES cells Smith and Hooper65 RRID:CVCL_Y481 Experimental models: Organisms/strains Mouse Igf2D(P1-P3) This paper N/A Mouse H19D13 Leighton et al.30 The Jackson Laboratory (Stock #036471) Mouse Igf2LacZ Murrell et al. 200135 N/A Mouse CMVCre Schwenk et al.66 The Jackson Laboratory (Stock #006054) Mouse Igf2fl/fl Hammerle et al.34 N/A Mouse Mir483KO This paper N/A Mouse Mir4835C This paper N/A Mouse iTgMir483 This paper N/A Oligonucleotides Primers used for genotyping, see Table S7 This paper Sigma Aldrich (Merck) TaqMan assays used for RT-qPCR, see Table S7 This paper Thermo Fisher Scientific Primers used for RT-qPCR, see Table S7 This paper Sigma Aldrich (Merck) Recombinant DNA pCAG-Cre plasmid Matsuda and Cepko67 RRID:Addgene_13775 pMA_F3NeoLoxPPolyFRT vector Prosser et al.64 N/A pPGKFLPobpA plasmid Raymond and Soriano68 RRID:Addgene_13793 pTET-BigT plasmid Mao et al.69 N/A pROSA26PAS plasmid Mao et al.69 N/A pGL3-Basic Promega (#E1751) RRID:Addgene_40342 Software and algorithms DGE analysis of the effect of Mir-483 on mouse livers This paper https://doi.org/10.5281/zenodo.
SYBR Green Assay:Article Title: Overexpression of Igf2-derived Mir483 inhibits Igf1 expression and leads to developmental growth restriction and metabolic dysfunction in mice.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-phospho-AKT (Ser 473) Cell Signaling Technology RRID:AB_329825 Polyclonal Goat anti-Mouse IGF-II R&D Systems RRID:AB_2122526 Polyclonal Goat anti-Mouse IGF-I R&D Systems RRID:AB_2248752 Rabbit polyclonal anti-SOD1 Abcam ab183881 Goat anti-Rabbit IgG (HRP) Abcam RRID:AB_955447 Rabbit anti-Goat IgG (HRP) ThermoFisher Scientific RRID:AB_228390 Recombinant Anti-Argonaute-2 antibody Abcam RRID:AB_2713978 Chemicals, peptides, and recombinant proteins Human LR3-IGF1 Preprotech 100-11R3 Protease inhibitors, set III, Calbiochem Merck 535140 Laemmli buffer Merck S3401 Laemmli lysis Merck 38733 RIPA lysis Merck R0278 Immobilon Forte Merck WBLUF Coomasie-250 staining Merck 1154440025 Critical commercial assays In-Fusion HD Cloning Plus kit Takara Bio 638909 RNeasy Plus Mini Kit Qiagen 74134 mirVana kit ThermoFisher Scientific AM1560 miRNeasy Mini kit Qiagen 217004 RNase-Free DNase ThermoFisher Scientific EN0521 RNA 6000 Pico kit Agilent 5067–1513 RNA 6000 Nano kit Agilent 5067–1511 DNA 12000 Kit Agilent 5067–1508 TruSeq Stranded mRNA HT Sample Prep Kit Illumina 20040532 TruSeq Stranded mRNA LT Sample Prep Kit Illumina RS-122-2101 TruSeq Small RNA Library Preparation Kit Illumina RS-200-0012 RevertAid RT Reverse Transcription Kit ThermoFisher Scientific K1622 TaqMan MicroRNA Reverse Transcription Kit ThermoFisher Scientific 4366596 SYBR Green JumpStart Taq Ready Mix Sigma Aldrich (Merck) S4438 TaqPath ProAmp Master Mix ThermoFisher Scientific A30866 TMTsixplex Isobaric Mass Tagging Kit ThermoFisher Scientific 90064 BCA assay ThermoFisher Scientific 23221 IGF-1 Quantikine ELISA kit Biotechne MG100 Mouse/rat growth hormone ELISA kit Millipore EZRMGH-45K Mouse IGF-II DuoSet ELISA kit R&D Systems DY792 Mouse/Rat Insulin Assay Kit Mercodia 10-1247-01 Roche’s Free Fatty Acid Kit Sigma Aldrich (Merck) 11383175001 QuickChange Site-Directed Mutagenesis kit Agilent Technologies 200518 Dual-Luciferase Reporter Assay System Promega E1910 Deposited data mRNA-seq obtained in E18.5 livers of the Mir483KO mouse model This paper GEO: GSE256302 (Continued on next page) 20 Cell Reports 43, 114750, September 24, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER mRNA-seq obtained in E10.5 embryos of the Mir4835C mouse model This paper GEO: GSE256306 mRNA-seq obtained in livers of 15-week-old Mir483KO mutants and littermate controls This paper GEO: GSE256304 miR-seq obtained in E18.5 livers of the Mir483KO mouse model This paper GEO: GSE256305 TMT mass spectrometry data obtained in E10.5 embryos of the Mir4835C mouse model This paper PXD051516 Experimental models: Cell lines E14 129ola male ES cells Hooper et al.63 RRID:CVCL_C320 Targeted JM8.F6 ES cells, International Knockout Mouse Consortium Project Design ID: 49935 Prosser et al.64 RRID:CVCL_J961 E14Tg2A.4 ES cells Smith and Hooper65 RRID:CVCL_Y481 Experimental models: Organisms/strains Mouse Igf2D(P1-P3) This paper N/A Mouse H19D13 Leighton et al.30 The Jackson Laboratory (Stock #036471) Mouse Igf2LacZ Murrell et al. 200135 N/A Mouse CMVCre Schwenk et al.66 The Jackson Laboratory (Stock #006054) Mouse Igf2fl/fl Hammerle et al.34 N/A Mouse Mir483KO This paper N/A Mouse Mir4835C This paper N/A Mouse iTgMir483 This paper N/A Oligonucleotides Primers used for genotyping, see Table S7 This paper Sigma Aldrich (Merck) TaqMan assays used for RT-qPCR, see Table S7 This paper Thermo Fisher Scientific Primers used for RT-qPCR, see Table S7 This paper Sigma Aldrich (Merck) Recombinant DNA pCAG-Cre plasmid Matsuda and Cepko67 RRID:Addgene_13775 pMA_F3NeoLoxPPolyFRT vector Prosser et al.64 N/A pPGKFLPobpA plasmid Raymond and Soriano68 RRID:Addgene_13793 pTET-BigT plasmid Mao et al.69 N/A pROSA26PAS plasmid Mao et al.69 N/A pGL3-Basic Promega (#E1751) RRID:Addgene_40342 Software and algorithms DGE analysis of the effect of Mir-483 on mouse livers This paper https://doi.org/10.5281/zenodo.
BIA-KA:Article Title: Overexpression of Igf2-derived Mir483 inhibits Igf1 expression and leads to developmental growth restriction and metabolic dysfunction in mice.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-phospho-AKT (Ser 473) Cell Signaling Technology RRID:AB_329825 Polyclonal Goat anti-Mouse IGF-II R&D Systems RRID:AB_2122526 Polyclonal Goat anti-Mouse IGF-I R&D Systems RRID:AB_2248752 Rabbit polyclonal anti-SOD1 Abcam ab183881 Goat anti-Rabbit IgG (HRP) Abcam RRID:AB_955447 Rabbit anti-Goat IgG (HRP) ThermoFisher Scientific RRID:AB_228390 Recombinant Anti-Argonaute-2 antibody Abcam RRID:AB_2713978 Chemicals, peptides, and recombinant proteins Human LR3-IGF1 Preprotech 100-11R3 Protease inhibitors, set III, Calbiochem Merck 535140 Laemmli buffer Merck S3401 Laemmli lysis Merck 38733 RIPA lysis Merck R0278 Immobilon Forte Merck WBLUF Coomasie-250 staining Merck 1154440025 Critical commercial assays In-Fusion HD Cloning Plus kit Takara Bio 638909 RNeasy Plus Mini Kit Qiagen 74134 mirVana kit ThermoFisher Scientific AM1560 miRNeasy Mini kit Qiagen 217004 RNase-Free DNase ThermoFisher Scientific EN0521 RNA 6000 Pico kit Agilent 5067–1513 RNA 6000 Nano kit Agilent 5067–1511 DNA 12000 Kit Agilent 5067–1508 TruSeq Stranded mRNA HT Sample Prep Kit Illumina 20040532 TruSeq Stranded mRNA LT Sample Prep Kit Illumina RS-122-2101 TruSeq Small RNA Library Preparation Kit Illumina RS-200-0012 RevertAid RT Reverse Transcription Kit ThermoFisher Scientific K1622 TaqMan MicroRNA Reverse Transcription Kit ThermoFisher Scientific 4366596 SYBR Green JumpStart Taq Ready Mix Sigma Aldrich (Merck) S4438 TaqPath ProAmp Master Mix ThermoFisher Scientific A30866 TMTsixplex Isobaric Mass Tagging Kit ThermoFisher Scientific 90064 BCA assay ThermoFisher Scientific 23221 IGF-1 Quantikine ELISA kit Biotechne MG100 Mouse/rat growth hormone ELISA kit Millipore EZRMGH-45K Mouse IGF-II DuoSet ELISA kit R&D Systems DY792 Mouse/Rat Insulin Assay Kit Mercodia 10-1247-01 Roche’s Free Fatty Acid Kit Sigma Aldrich (Merck) 11383175001 QuickChange Site-Directed Mutagenesis kit Agilent Technologies 200518 Dual-Luciferase Reporter Assay System Promega E1910 Deposited data mRNA-seq obtained in E18.5 livers of the Mir483KO mouse model This paper GEO: GSE256302 (Continued on next page) 20 Cell Reports 43, 114750, September 24, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER mRNA-seq obtained in E10.5 embryos of the Mir4835C mouse model This paper GEO: GSE256306 mRNA-seq obtained in livers of 15-week-old Mir483KO mutants and littermate controls This paper GEO: GSE256304 miR-seq obtained in E18.5 livers of the Mir483KO mouse model This paper GEO: GSE256305 TMT mass spectrometry data obtained in E10.5 embryos of the Mir4835C mouse model This paper PXD051516 Experimental models: Cell lines E14 129ola male ES cells Hooper et al.63 RRID:CVCL_C320 Targeted JM8.F6 ES cells, International Knockout Mouse Consortium Project Design ID: 49935 Prosser et al.64 RRID:CVCL_J961 E14Tg2A.4 ES cells Smith and Hooper65 RRID:CVCL_Y481 Experimental models: Organisms/strains Mouse Igf2D(P1-P3) This paper N/A Mouse H19D13 Leighton et al.30 The Jackson Laboratory (Stock #036471) Mouse Igf2LacZ Murrell et al. 200135 N/A Mouse CMVCre Schwenk et al.66 The Jackson Laboratory (Stock #006054) Mouse Igf2fl/fl Hammerle et al.34 N/A Mouse Mir483KO This paper N/A Mouse Mir4835C This paper N/A Mouse iTgMir483 This paper N/A Oligonucleotides Primers used for genotyping, see Table S7 This paper Sigma Aldrich (Merck) TaqMan assays used for RT-qPCR, see Table S7 This paper Thermo Fisher Scientific Primers used for RT-qPCR, see Table S7 This paper Sigma Aldrich (Merck) Recombinant DNA pCAG-Cre plasmid Matsuda and Cepko67 RRID:Addgene_13775 pMA_F3NeoLoxPPolyFRT vector Prosser et al.64 N/A pPGKFLPobpA plasmid Raymond and Soriano68 RRID:Addgene_13793 pTET-BigT plasmid Mao et al.69 N/A pROSA26PAS plasmid Mao et al.69 N/A pGL3-Basic Promega (#E1751) RRID:Addgene_40342 Software and algorithms DGE analysis of the effect of Mir-483 on mouse livers This paper https://doi.org/10.5281/zenodo.
Enzyme-linked Immunosorbent Assay:Article Title: Overexpression of Igf2-derived Mir483 inhibits Igf1 expression and leads to developmental growth restriction and metabolic dysfunction in mice.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-phospho-AKT (Ser 473) Cell Signaling Technology RRID:AB_329825 Polyclonal Goat anti-Mouse IGF-II R&D Systems RRID:AB_2122526 Polyclonal Goat anti-Mouse IGF-I R&D Systems RRID:AB_2248752 Rabbit polyclonal anti-SOD1 Abcam ab183881 Goat anti-Rabbit IgG (HRP) Abcam RRID:AB_955447 Rabbit anti-Goat IgG (HRP) ThermoFisher Scientific RRID:AB_228390 Recombinant Anti-Argonaute-2 antibody Abcam RRID:AB_2713978 Chemicals, peptides, and recombinant proteins Human LR3-IGF1 Preprotech 100-11R3 Protease inhibitors, set III, Calbiochem Merck 535140 Laemmli buffer Merck S3401 Laemmli lysis Merck 38733 RIPA lysis Merck R0278 Immobilon Forte Merck WBLUF Coomasie-250 staining Merck 1154440025 Critical commercial assays In-Fusion HD Cloning Plus kit Takara Bio 638909 RNeasy Plus Mini Kit Qiagen 74134 mirVana kit ThermoFisher Scientific AM1560 miRNeasy Mini kit Qiagen 217004 RNase-Free DNase ThermoFisher Scientific EN0521 RNA 6000 Pico kit Agilent 5067–1513 RNA 6000 Nano kit Agilent 5067–1511 DNA 12000 Kit Agilent 5067–1508 TruSeq Stranded mRNA HT Sample Prep Kit Illumina 20040532 TruSeq Stranded mRNA LT Sample Prep Kit Illumina RS-122-2101 TruSeq Small RNA Library Preparation Kit Illumina RS-200-0012 RevertAid RT Reverse Transcription Kit ThermoFisher Scientific K1622 TaqMan MicroRNA Reverse Transcription Kit ThermoFisher Scientific 4366596 SYBR Green JumpStart Taq Ready Mix Sigma Aldrich (Merck) S4438 TaqPath ProAmp Master Mix ThermoFisher Scientific A30866 TMTsixplex Isobaric Mass Tagging Kit ThermoFisher Scientific 90064 BCA assay ThermoFisher Scientific 23221 IGF-1 Quantikine ELISA kit Biotechne MG100 Mouse/rat growth hormone ELISA kit Millipore EZRMGH-45K Mouse IGF-II DuoSet ELISA kit R&D Systems DY792 Mouse/Rat Insulin Assay Kit Mercodia 10-1247-01 Roche’s Free Fatty Acid Kit Sigma Aldrich (Merck) 11383175001 QuickChange Site-Directed Mutagenesis kit Agilent Technologies 200518 Dual-Luciferase Reporter Assay System Promega E1910 Deposited data mRNA-seq obtained in E18.5 livers of the Mir483KO mouse model This paper GEO: GSE256302 (Continued on next page) 20 Cell Reports 43, 114750, September 24, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER mRNA-seq obtained in E10.5 embryos of the Mir4835C mouse model This paper GEO: GSE256306 mRNA-seq obtained in livers of 15-week-old Mir483KO mutants and littermate controls This paper GEO: GSE256304 miR-seq obtained in E18.5 livers of the Mir483KO mouse model This paper GEO: GSE256305 TMT mass spectrometry data obtained in E10.5 embryos of the Mir4835C mouse model This paper PXD051516 Experimental models: Cell lines E14 129ola male ES cells Hooper et al.63 RRID:CVCL_C320 Targeted JM8.F6 ES cells, International Knockout Mouse Consortium Project Design ID: 49935 Prosser et al.64 RRID:CVCL_J961 E14Tg2A.4 ES cells Smith and Hooper65 RRID:CVCL_Y481 Experimental models: Organisms/strains Mouse Igf2D(P1-P3) This paper N/A Mouse H19D13 Leighton et al.30 The Jackson Laboratory (Stock #036471) Mouse Igf2LacZ Murrell et al. 200135 N/A Mouse CMVCre Schwenk et al.66 The Jackson Laboratory (Stock #006054) Mouse Igf2fl/fl Hammerle et al.34 N/A Mouse Mir483KO This paper N/A Mouse Mir4835C This paper N/A Mouse iTgMir483 This paper N/A Oligonucleotides Primers used for genotyping, see Table S7 This paper Sigma Aldrich (Merck) TaqMan assays used for RT-qPCR, see Table S7 This paper Thermo Fisher Scientific Primers used for RT-qPCR, see Table S7 This paper Sigma Aldrich (Merck) Recombinant DNA pCAG-Cre plasmid Matsuda and Cepko67 RRID:Addgene_13775 pMA_F3NeoLoxPPolyFRT vector Prosser et al.64 N/A pPGKFLPobpA plasmid Raymond and Soriano68 RRID:Addgene_13793 pTET-BigT plasmid Mao et al.69 N/A pROSA26PAS plasmid Mao et al.69 N/A pGL3-Basic Promega (#E1751) RRID:Addgene_40342 Software and algorithms DGE analysis of the effect of Mir-483 on mouse livers This paper https://doi.org/10.5281/zenodo.
Mutagenesis:Article Title: Overexpression of Igf2-derived Mir483 inhibits Igf1 expression and leads to developmental growth restriction and metabolic dysfunction in mice.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-phospho-AKT (Ser 473) Cell Signaling Technology RRID:AB_329825 Polyclonal Goat anti-Mouse IGF-II R&D Systems RRID:AB_2122526 Polyclonal Goat anti-Mouse IGF-I R&D Systems RRID:AB_2248752 Rabbit polyclonal anti-SOD1 Abcam ab183881 Goat anti-Rabbit IgG (HRP) Abcam RRID:AB_955447 Rabbit anti-Goat IgG (HRP) ThermoFisher Scientific RRID:AB_228390 Recombinant Anti-Argonaute-2 antibody Abcam RRID:AB_2713978 Chemicals, peptides, and recombinant proteins Human LR3-IGF1 Preprotech 100-11R3 Protease inhibitors, set III, Calbiochem Merck 535140 Laemmli buffer Merck S3401 Laemmli lysis Merck 38733 RIPA lysis Merck R0278 Immobilon Forte Merck WBLUF Coomasie-250 staining Merck 1154440025 Critical commercial assays In-Fusion HD Cloning Plus kit Takara Bio 638909 RNeasy Plus Mini Kit Qiagen 74134 mirVana kit ThermoFisher Scientific AM1560 miRNeasy Mini kit Qiagen 217004 RNase-Free DNase ThermoFisher Scientific EN0521 RNA 6000 Pico kit Agilent 5067–1513 RNA 6000 Nano kit Agilent 5067–1511 DNA 12000 Kit Agilent 5067–1508 TruSeq Stranded mRNA HT Sample Prep Kit Illumina 20040532 TruSeq Stranded mRNA LT Sample Prep Kit Illumina RS-122-2101 TruSeq Small RNA Library Preparation Kit Illumina RS-200-0012 RevertAid RT Reverse Transcription Kit ThermoFisher Scientific K1622 TaqMan MicroRNA Reverse Transcription Kit ThermoFisher Scientific 4366596 SYBR Green JumpStart Taq Ready Mix Sigma Aldrich (Merck) S4438 TaqPath ProAmp Master Mix ThermoFisher Scientific A30866 TMTsixplex Isobaric Mass Tagging Kit ThermoFisher Scientific 90064 BCA assay ThermoFisher Scientific 23221 IGF-1 Quantikine ELISA kit Biotechne MG100 Mouse/rat growth hormone ELISA kit Millipore EZRMGH-45K Mouse IGF-II DuoSet ELISA kit R&D Systems DY792 Mouse/Rat Insulin Assay Kit Mercodia 10-1247-01 Roche’s Free Fatty Acid Kit Sigma Aldrich (Merck) 11383175001 QuickChange Site-Directed Mutagenesis kit Agilent Technologies 200518 Dual-Luciferase Reporter Assay System Promega E1910 Deposited data mRNA-seq obtained in E18.5 livers of the Mir483KO mouse model This paper GEO: GSE256302 (Continued on next page) 20 Cell Reports 43, 114750, September 24, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER mRNA-seq obtained in E10.5 embryos of the Mir4835C mouse model This paper GEO: GSE256306 mRNA-seq obtained in livers of 15-week-old Mir483KO mutants and littermate controls This paper GEO: GSE256304 miR-seq obtained in E18.5 livers of the Mir483KO mouse model This paper GEO: GSE256305 TMT mass spectrometry data obtained in E10.5 embryos of the Mir4835C mouse model This paper PXD051516 Experimental models: Cell lines E14 129ola male ES cells Hooper et al.63 RRID:CVCL_C320 Targeted JM8.F6 ES cells, International Knockout Mouse Consortium Project Design ID: 49935 Prosser et al.64 RRID:CVCL_J961 E14Tg2A.4 ES cells Smith and Hooper65 RRID:CVCL_Y481 Experimental models: Organisms/strains Mouse Igf2D(P1-P3) This paper N/A Mouse H19D13 Leighton et al.30 The Jackson Laboratory (Stock #036471) Mouse Igf2LacZ Murrell et al. 200135 N/A Mouse CMVCre Schwenk et al.66 The Jackson Laboratory (Stock #006054) Mouse Igf2fl/fl Hammerle et al.34 N/A Mouse Mir483KO This paper N/A Mouse Mir4835C This paper N/A Mouse iTgMir483 This paper N/A Oligonucleotides Primers used for genotyping, see Table S7 This paper Sigma Aldrich (Merck) TaqMan assays used for RT-qPCR, see Table S7 This paper Thermo Fisher Scientific Primers used for RT-qPCR, see Table S7 This paper Sigma Aldrich (Merck) Recombinant DNA pCAG-Cre plasmid Matsuda and Cepko67 RRID:Addgene_13775 pMA_F3NeoLoxPPolyFRT vector Prosser et al.64 N/A pPGKFLPobpA plasmid Raymond and Soriano68 RRID:Addgene_13793 pTET-BigT plasmid Mao et al.69 N/A pROSA26PAS plasmid Mao et al.69 N/A pGL3-Basic Promega (#E1751) RRID:Addgene_40342 Software and algorithms DGE analysis of the effect of Mir-483 on mouse livers This paper https://doi.org/10.5281/zenodo.
Reporter Assay:Article Title: Overexpression of Igf2-derived Mir483 inhibits Igf1 expression and leads to developmental growth restriction and metabolic dysfunction in mice.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-phospho-AKT (Ser 473) Cell Signaling Technology RRID:AB_329825 Polyclonal Goat anti-Mouse IGF-II R&D Systems RRID:AB_2122526 Polyclonal Goat anti-Mouse IGF-I R&D Systems RRID:AB_2248752 Rabbit polyclonal anti-SOD1 Abcam ab183881 Goat anti-Rabbit IgG (HRP) Abcam RRID:AB_955447 Rabbit anti-Goat IgG (HRP) ThermoFisher Scientific RRID:AB_228390 Recombinant Anti-Argonaute-2 antibody Abcam RRID:AB_2713978 Chemicals, peptides, and recombinant proteins Human LR3-IGF1 Preprotech 100-11R3 Protease inhibitors, set III, Calbiochem Merck 535140 Laemmli buffer Merck S3401 Laemmli lysis Merck 38733 RIPA lysis Merck R0278 Immobilon Forte Merck WBLUF Coomasie-250 staining Merck 1154440025 Critical commercial assays In-Fusion HD Cloning Plus kit Takara Bio 638909 RNeasy Plus Mini Kit Qiagen 74134 mirVana kit ThermoFisher Scientific AM1560 miRNeasy Mini kit Qiagen 217004 RNase-Free DNase ThermoFisher Scientific EN0521 RNA 6000 Pico kit Agilent 5067–1513 RNA 6000 Nano kit Agilent 5067–1511 DNA 12000 Kit Agilent 5067–1508 TruSeq Stranded mRNA HT Sample Prep Kit Illumina 20040532 TruSeq Stranded mRNA LT Sample Prep Kit Illumina RS-122-2101 TruSeq Small RNA Library Preparation Kit Illumina RS-200-0012 RevertAid RT Reverse Transcription Kit ThermoFisher Scientific K1622 TaqMan MicroRNA Reverse Transcription Kit ThermoFisher Scientific 4366596 SYBR Green JumpStart Taq Ready Mix Sigma Aldrich (Merck) S4438 TaqPath ProAmp Master Mix ThermoFisher Scientific A30866 TMTsixplex Isobaric Mass Tagging Kit ThermoFisher Scientific 90064 BCA assay ThermoFisher Scientific 23221 IGF-1 Quantikine ELISA kit Biotechne MG100 Mouse/rat growth hormone ELISA kit Millipore EZRMGH-45K Mouse IGF-II DuoSet ELISA kit R&D Systems DY792 Mouse/Rat Insulin Assay Kit Mercodia 10-1247-01 Roche’s Free Fatty Acid Kit Sigma Aldrich (Merck) 11383175001 QuickChange Site-Directed Mutagenesis kit Agilent Technologies 200518 Dual-Luciferase Reporter Assay System Promega E1910 Deposited data mRNA-seq obtained in E18.5 livers of the Mir483KO mouse model This paper GEO: GSE256302 (Continued on next page) 20 Cell Reports 43, 114750, September 24, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER mRNA-seq obtained in E10.5 embryos of the Mir4835C mouse model This paper GEO: GSE256306 mRNA-seq obtained in livers of 15-week-old Mir483KO mutants and littermate controls This paper GEO: GSE256304 miR-seq obtained in E18.5 livers of the Mir483KO mouse model This paper GEO: GSE256305 TMT mass spectrometry data obtained in E10.5 embryos of the Mir4835C mouse model This paper PXD051516 Experimental models: Cell lines E14 129ola male ES cells Hooper et al.63 RRID:CVCL_C320 Targeted JM8.F6 ES cells, International Knockout Mouse Consortium Project Design ID: 49935 Prosser et al.64 RRID:CVCL_J961 E14Tg2A.4 ES cells Smith and Hooper65 RRID:CVCL_Y481 Experimental models: Organisms/strains Mouse Igf2D(P1-P3) This paper N/A Mouse H19D13 Leighton et al.30 The Jackson Laboratory (Stock #036471) Mouse Igf2LacZ Murrell et al. 200135 N/A Mouse CMVCre Schwenk et al.66 The Jackson Laboratory (Stock #006054) Mouse Igf2fl/fl Hammerle et al.34 N/A Mouse Mir483KO This paper N/A Mouse Mir4835C This paper N/A Mouse iTgMir483 This paper N/A Oligonucleotides Primers used for genotyping, see Table S7 This paper Sigma Aldrich (Merck) TaqMan assays used for RT-qPCR, see Table S7 This paper Thermo Fisher Scientific Primers used for RT-qPCR, see Table S7 This paper Sigma Aldrich (Merck) Recombinant DNA pCAG-Cre plasmid Matsuda and Cepko67 RRID:Addgene_13775 pMA_F3NeoLoxPPolyFRT vector Prosser et al.64 N/A pPGKFLPobpA plasmid Raymond and Soriano68 RRID:Addgene_13793 pTET-BigT plasmid Mao et al.69 N/A pROSA26PAS plasmid Mao et al.69 N/A pGL3-Basic Promega (#E1751) RRID:Addgene_40342 Software and algorithms DGE analysis of the effect of Mir-483 on mouse livers This paper https://doi.org/10.5281/zenodo.
|
Cell Signaling Technology Inc is a verified supplier 
Cell Signaling Technology Inc manufactures this product 